Hotspot

rpl33-rps18

LSC · 23639-24171 · 533 bp

Back to Mimosa

Pi 0.153
MarkerSeek score 76.90
Primer available yes
Label rpl33-rps18

Feature Metadata

label_name
rpl33-rps18
feature_type
igs
region
LSC
length_bp
533
variable_sites
93
pi
0.153
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.66
markerseek_score
76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl33-rps18_p1 TCTGTCCCTGTTGTTGCAAA GGGCAAACGTCTACGAAAAG 45.0 50.0 57.9 57.4 433-643 bp 1.0 86.2
rpl33-rps18_p2 TTCTGTCCCTGTTGTTGCAA GGGCAAACGTCTACGAAAAG 45.0 50.0 57.9 57.4 434-644 bp 1.0 86.2
rpl33-rps18_p3 TCTGTCCCTGTTGTTGCAAA GGGGCAAACGTCTACGAAAA 45.0 50.0 57.9 58.8 434-644 bp 1.0 84.7
rpl33-rps18_p4 TTCTGTCCCTGTTGTTGCAA GGGGCAAACGTCTACGAAAA 45.0 50.0 57.9 58.8 435-645 bp 1.0 84.7
rpl33-rps18_p5 TCTGTCCCTGTTGTTGCAAA GGGGCAAACGTCTACGAAA 45.0 52.6 57.9 58.1 434-644 bp 1.0 82.2