Hotspot

rbcL-accD

LSC · 11351-12417 · 1067 bp

Back to Mimosa

Pi 0.0762
MarkerSeek score 83.60
Primer available yes
Label rbcL-accD

Feature Metadata

label_name
rbcL-accD
feature_type
igs
region
LSC
length_bp
1067
variable_sites
134
pi
0.0762
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.91
markerseek_score
83.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-accD_p1 GAGCTGTATTTGCCCGAGAA TTTGGTCGTCACTTCCACTG 50.0 50.0 58.0 58.1 1859 bp 0.25 49.4
rbcL-accD_p2 AAGAGCTGTATTTGCCCGAG TTTGGTCGTCACTTCCACTG 50.0 50.0 58.0 58.1 1861 bp 0.25 49.3
rbcL-accD_p3 AGTCCTGAATTAGCTGCTGC TTTGGTCGTCACTTCCACTG 50.0 50.0 58.0 58.1 1263 bp 0.25 49.3
rbcL-accD_p4 GAGGCTAGCAAATGGAGTCC TTTGGTCGTCACTTCCACTG 55.0 50.0 58.0 58.1 1278 bp 0.25 49.3
rbcL-accD_p5 CGCTGGTACCGTAGTAGGTA TTTGGTCGTCACTTCCACTG 55.0 50.0 58.0 58.1 1636 bp 0.25 49.2