Hotspot

trnL-UAA-trnF-GAA

LSC · 1757-2190 · 434 bp

Back to Mimosa

Pi 0.0524
MarkerSeek score 81.90
Primer available yes
Label trnL-UAA-trnF-GAA

Feature Metadata

label_name
trnL-UAA-trnF-GAA
feature_type
igs
region
LSC
length_bp
434
variable_sites
42
pi
0.0524
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.94
markerseek_score
81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAA-trnF-GAA_p1 AATCGTGAGGGTTCAAGTCC TCTACCAGCTGAGCTATCCC 50.0 55.0 57.5 58.0 460-488 bp 1.0 87.3
trnL-UAA-trnF-GAA_p2 AATCGTGAGGGTTCAAGTCC TCAGTCCTCTGCTCTACCAG 50.0 55.0 57.5 57.9 472-500 bp 1.0 86.1
trnL-UAA-trnF-GAA_p3 TTCAAGTCCCTCTATCCCCA TCTACCAGCTGAGCTATCCC 50.0 55.0 57.0 58.0 449-477 bp 1.0 83.6
trnL-UAA-trnF-GAA_p4 AATCGTGAGGGTTCAAGTCC TTCAGTCCTCTGCTCTACCA 50.0 50.0 57.5 57.4 473-501 bp 1.0 82.3
trnL-UAA-trnF-GAA_p5 TTCAAGTCCCTCTATCCCCA TCAGTCCTCTGCTCTACCAG 50.0 55.0 57.0 57.9 461-489 bp 1.0 82.3