Hotspot

ycf1

SSC · 123685-129060 · 5376 bp

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Pi 0.00691

MarkerSeek score 43.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5376
variable_sites: 37
pi: 0.00691
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGAAGTCCCCTTTTCTTTTCTTCT	AAAGATAAATTGAGTGAAAGCA	37.5	27.3	58.0	52.3	206 bp	1.0	42.7
ycf1_p2	AGAAGTCCCCTTTTCTTTTCTTCT	AAGATAAATTGAGTGAAAGCAA	37.5	27.3	58.0	52.3	205 bp	1.0	42.7
ycf1_p3	AGAAGTCCCCTTTTCTTTTCTTCT	AGATAAATTGAGTGAAAGCAAA	37.5	27.3	58.0	52.3	204 bp	1.0	42.7
ycf1_p4	TCTCTGAAATTAGAAGTCCCCT	AAGATAAATTGAGTGAAAGCAA	40.9	27.3	55.9	52.3	216 bp	1.0	42.6
ycf1_p5	TCTCTGAAATTAGAAGTCCCCT	AGATAAATTGAGTGAAAGCAAA	40.9	27.3	55.9	52.3	215 bp	1.0	42.6