Hotspot

rps16

LSC · 56335-57485 · 1151 bp

Back to Millettia

Pi 0.00962

MarkerSeek score 64.90

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1151
variable_sites: 11
pi: 0.00962
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	TAGAACAAGAGAGCTGGGGG	ATCCAAGCTAAGGGAATGTT	55.0	40.0	58.4	54.3	1255-1257 bp	1.0	47.8
rps16_p2	TAGAACAAGAGAGCTGGGGG	TATCCAAGCTAAGGGAATGT	55.0	40.0	58.4	53.4	1256-1258 bp	1.0	41.1
rps16_p3	TAGAACAAGAGAGCTGGGGG	TATCCAAGCTAAGGGAATGTT	55.0	38.1	58.4	54.3	1256-1258 bp	1.0	41.1
rps16_p4	AGAACAAGAGAGCTGGGGG	ATCCAAGCTAAGGGAATGTT	57.9	40.0	58.6	54.3	1254-1256 bp	1.0	41.1
rps16_p5	TAGAACAAGAGAGCTGGGGG	ATATCCAAGCTAAGGGAATGT	55.0	38.1	58.4	54.0	1257-1259 bp	1.0	41.1