Hotspot

rpl33

LSC · 67727-67927 · 201 bp

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Pi 0.00995

MarkerSeek score 47.30

Primer available yes

Label rpl33

Feature Metadata

label_name: rpl33
feature_type: gene
region: LSC
length_bp: 201
variable_sites: 2
pi: 0.00995
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33_p1	CCTTTCTTCGCTTCAAATCCG	GTTATGCCCCTTGGTTGGAA	47.6	50.0	57.9	58.1	311 bp	1.0	71.0
rpl33_p2	CCTTTCTTCGCTTCAAATCCG	ATGTTATGCCCCTTGGTTGG	47.6	50.0	57.9	58.1	313 bp	1.0	70.4
rpl33_p3	CCTTTCTTCGCTTCAAATCCG	TATGCCCCTTGGTTGGAAAG	47.6	50.0	57.9	57.8	309 bp	1.0	69.7
rpl33_p4	CCTTTCTTCGCTTCAAATCCG	CCCCTTGGTTGGAAAGGTAA	47.6	50.0	57.9	57.3	305 bp	1.0	66.0
rpl33_p5	CCTTTCTTCGCTTCAAATCCG	TTATGCCCCTTGGTTGGAAA	47.6	45.0	57.9	57.3	310 bp	1.0	65.9