Hotspot

ndhF

SSC · 110411-112657 · 2247 bp

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Pi 0.00935

MarkerSeek score 47.60

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2247
variable_sites: 21
pi: 0.00935
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ATCGTTGTGGTCGGACTCTA	TGGAAGTATTGTGCAAAGATTCAG	50.0	37.5	58.2	57.7	2811-2812 bp	1.0	45.3
ndhF_p2	TCGTTGTGGTCGGACTCTAT	TGGAAGTATTGTGCAAAGATTCAG	50.0	37.5	58.2	57.7	2810-2811 bp	1.0	45.3
ndhF_p3	ATCGTTGTGGTCGGACTCTA	TGGAAGTATTGTGCAAAGATTCA	50.0	34.8	58.2	56.7	2811-2812 bp	1.0	45.0
ndhF_p4	TCGTTGTGGTCGGACTCTAT	TGGAAGTATTGTGCAAAGATTCA	50.0	34.8	58.2	56.7	2810-2811 bp	1.0	45.0
ndhF_p5	CATTCTCCATAGGGCCCTCT	ACGAATGTTTTTAGTTTTGGTGGA	55.0	33.3	58.3	57.8	2994-2997 bp	1.0	45.0