Hotspot

ccsA

SSC · 113987-114961 · 975 bp

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Pi 0.00821
MarkerSeek score 52.20
Primer available yes
Label ccsA

Feature Metadata

label_name
ccsA
feature_type
gene
region
SSC
length_bp
975
variable_sites
8
pi
0.00821
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA_p1 TGAACTCAATTCCTATTTCTATTCCT TGCTTTCTATATTTGTATTTGTGAAGT 30.8 25.9 56.2 56.1 1068-1069 bp 1.0 40.6
ccsA_p2 TGAACTCAATTCCTATTTCTATTCC TGCTTTCTATATTTGTATTTGTGAAGT 32.0 25.9 54.9 56.1 1068-1069 bp 1.0 40.6
ccsA_p3 TGAACTCAATTCCTATTTCTATTCCT TGCTTTCTATATTTGTATTTGTGA 30.8 25.0 56.2 52.8 1068-1069 bp 1.0 40.6
ccsA_p4 TGAACTCAATTCCTATTTCTATTCCT TGCTTTCTATATTTGTATTTGTGAAG 30.8 26.9 56.2 54.7 1068-1069 bp 1.0 40.6
ccsA_p5 TGAACTCAATTCCTATTTCTATTCCT GCTTTCTATATTTGTATTTGTGAAGT 30.8 26.9 56.2 54.7 1067-1068 bp 1.0 40.6