Hotspot

trnL-ccsA

SSC · 113893-113986 · 94 bp

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Pi 0.0638

MarkerSeek score 72.50

Primer available yes

Label trnL-ccsA

Feature Metadata

label_name: trnL-ccsA
feature_type: igs
region: SSC
length_bp: 94
variable_sites: 6
pi: 0.0638
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-ccsA_p1	TAGACACGCTGCTCTTAGGA	ATTGATACGATCGAAAACGA	50.0	35.0	57.9	52.9	214-216 bp	1.0	42.2
trnL-ccsA_p2	TAGACACGCTGCTCTTAGGA	TTGATACGATCGAAAACGAT	50.0	35.0	57.9	52.9	213-215 bp	1.0	42.2
trnL-ccsA_p3	CGAATAAGCCGCTATGGTGA	ATTGATACGATCGAAAACGA	50.0	35.0	57.9	52.9	240-242 bp	1.0	42.0
trnL-ccsA_p4	CGAATAAGCCGCTATGGTGA	TTGATACGATCGAAAACGAT	50.0	35.0	57.9	52.9	239-241 bp	1.0	42.0
trnL-ccsA_p5	TCGAATAAGCCGCTATGGTG	TTGATACGATCGAAAACGAT	50.0	35.0	57.9	52.9	240-242 bp	1.0	42.0