Hotspot

ycf1-ndhF

IRb · 110402-110410 · 9 bp

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Pi 0.222

MarkerSeek score 76.30

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: IRb
length_bp: 9
variable_sites: 2
pi: 0.222
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	CGTGGAACTTCTTCGCAGTA	TATTCGTTGGGGCCATAGGA	50.0	50.0	57.9	58.2	1286 bp	1.0	78.7
ycf1-ndhF_p2	TGGAACTTCTTCGCAGTAGC	TATTCGTTGGGGCCATAGGA	50.0	50.0	57.9	58.2	1284 bp	1.0	78.5
ycf1-ndhF_p3	CGTGGAACTTCTTCGCAGTA	CGTTGGGGCCATAGGAATTT	50.0	50.0	57.9	58.2	1282 bp	1.0	78.4
ycf1-ndhF_p4	GTAGCTCCATTCCATGCCTC	TATTCGTTGGGGCCATAGGA	55.0	50.0	58.1	58.2	1269 bp	1.0	78.3
ycf1-ndhF_p5	TGGAACTTCTTCGCAGTAGC	CGTTGGGGCCATAGGAATTT	50.0	50.0	57.9	58.2	1280 bp	1.0	78.2