Hotspot

rps8-rpl14

LSC · 80854-81338 · 485 bp

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Pi 0.0236

MarkerSeek score 63.60

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 485
variable_sites: 11
pi: 0.0236
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	CAGCGATGGTGTCCTTACTC	GTTTCATTAGCACCCGAGGT	55.0	50.0	58.1	57.9	516-534 bp	1.0	83.0
rps8-rpl14_p2	TTATGTCAGCGATGGTGTCC	GTTTCATTAGCACCCGAGGT	50.0	50.0	57.7	57.9	522-540 bp	1.0	81.0
rps8-rpl14_p3	GTCAGCGATGGTGTCCTTAC	GTTTCATTAGCACCCGAGGT	55.0	50.0	58.4	57.9	518-536 bp	1.0	80.7
rps8-rpl14_p4	CAGCGATGGTGTCCTTACTC	AGTTTCATTAGCACCCGAGG	55.0	50.0	58.1	57.6	517-535 bp	1.0	80.6
rps8-rpl14_p5	AGCGATGGTGTCCTTACTCA	GTTTCATTAGCACCCGAGGT	50.0	50.0	58.4	57.9	515-533 bp	1.0	80.0