Hotspot

rpl33-rps18

LSC · 67928-68102 · 175 bp

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Pi 0.0571

MarkerSeek score 72.40

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 175
variable_sites: 10
pi: 0.0571
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	TCTGTCCCTGTTGTTGCAAA	TCAATTTGGTCCCCCGATTG	45.0	50.0	57.9	58.2	311-312 bp	1.0	80.2
rpl33-rps18_p2	TTCTGTCCCTGTTGTTGCAA	TCAATTTGGTCCCCCGATTG	45.0	50.0	57.9	58.2	312-313 bp	1.0	80.2
rpl33-rps18_p3	ATTCTGTCCCTGTTGTTGCA	TCAATTTGGTCCCCCGATTG	45.0	50.0	57.6	58.2	313-314 bp	1.0	78.4
rpl33-rps18_p4	TCTGTCCCTGTTGTTGCAAA	AATCAATTTGGTCCCCCGAT	45.0	45.0	57.9	57.2	313-314 bp	1.0	74.7
rpl33-rps18_p5	TCTGTCCCTGTTGTTGCAAA	ATCAATTTGGTCCCCCGATT	45.0	45.0	57.9	57.2	312-313 bp	1.0	74.7