Hotspot

trnP-psaJ

LSC · 66739-67111 · 373 bp

Back to Millettia

Pi 0.0215

MarkerSeek score 56.30

Primer available yes

Label trnP-psaJ

Feature Metadata

label_name: trnP-psaJ
feature_type: igs
region: LSC
length_bp: 373
variable_sites: 8
pi: 0.0215
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-psaJ_p1	TACCAAACTGCGCTACATCC	TGGTGCCACGGAAAGATATG	50.0	50.0	58.0	58.0	431-438 bp	1.0	81.2
trnP-psaJ_p2	TACCAAACTGCGCTACATCC	GGTGCCACGGAAAGATATGT	50.0	50.0	58.0	58.0	430-437 bp	1.0	81.2
trnP-psaJ_p3	ACCCGTGACATTTTGTACCC	TGGTGCCACGGAAAGATATG	50.0	50.0	58.1	58.0	464-471 bp	1.0	80.4
trnP-psaJ_p4	CCCGTGACATTTTGTACCCA	TGGTGCCACGGAAAGATATG	50.0	50.0	58.1	58.0	463-470 bp	1.0	80.4
trnP-psaJ_p5	ACCCGTGACATTTTGTACCC	GGTGCCACGGAAAGATATGT	50.0	50.0	58.1	58.0	463-470 bp	1.0	80.4