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psbE-petL

LSC · 64592-65937 · 1346 bp

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Pi 0.00823

MarkerSeek score 64.20

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1346
variable_sites: 11
pi: 0.00823
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	AACCGCTAGTAGAAAACCGA	50.0	45.0	57.8	56.6	1415-1430 bp	1.0	68.7
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	ACCGCTAGTAGAAAACCGAA	50.0	45.0	57.8	56.6	1414-1429 bp	1.0	68.7
psbE-petL_p3	GTTCTCCTGTGCTTCCAGAC	AACCGCTAGTAGAAAACCGA	55.0	45.0	58.2	56.6	1410-1425 bp	1.0	68.4
psbE-petL_p4	GTTCTCCTGTGCTTCCAGAC	ACCGCTAGTAGAAAACCGAA	55.0	45.0	58.2	56.6	1409-1424 bp	1.0	68.4
psbE-petL_p5	TCTCCTGTGCTTCCAGACAT	ACCGCTAGTAGAAAACCGAA	50.0	45.0	58.4	56.6	1407-1422 bp	1.0	67.2