Hotspot

trnQ-psbK

LSC · 58055-58525 · 471 bp

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Pi 0.00651

MarkerSeek score 60.90

Primer available yes

Label trnQ-psbK

Feature Metadata

label_name: trnQ-psbK
feature_type: igs
region: LSC
length_bp: 471
variable_sites: 3
pi: 0.00651
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-psbK_p1	TGGATATGTTCTGGGACGGA	CGTAGGCTTCAGGCAATTTC	50.0	50.0	57.8	57.2	642-644 bp	1.0	73.6
trnQ-psbK_p2	TGGATATGTTCTGGGACGGA	GTAGGCTTCAGGCAATTTCG	50.0	50.0	57.8	57.2	641-643 bp	1.0	73.6
trnQ-psbK_p3	TGGATATGTTCTGGGACGGA	TAGGCTTCAGGCAATTTCGC	50.0	50.0	57.8	58.9	640-642 bp	1.0	73.0
trnQ-psbK_p4	TGGATATGTTCTGGGACGGA	GCGTAGGCTTCAGGCAATTT	50.0	50.0	57.8	59.2	643-645 bp	1.0	70.8
trnQ-psbK_p5	CTGGATATGTTCTGGGACGG	GTAGGCTTCAGGCAATTTCG	55.0	50.0	57.5	57.2	642-644 bp	1.0	70.7