Hotspot

trnK-rbcL

LSC · 4388-5034 · 647 bp

Back to Millettia

Pi 0.013
MarkerSeek score 71.00
Primer available yes
Label trnK-rbcL

Feature Metadata

label_name
trnK-rbcL
feature_type
igs
region
LSC
length_bp
647
variable_sites
8
pi
0.013
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
71.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-rbcL_p1 AGTCGCTCTTAAAAGCCGAG AGTCCTGAATTAGCTGCTGC 50.0 50.0 58.0 58.0 739-766 bp 1.0 84.1
trnK-rbcL_p2 GAGTCGCTCTTAAAAGCCGA AGTCCTGAATTAGCTGCTGC 50.0 50.0 58.0 58.0 740-767 bp 1.0 84.0
trnK-rbcL_p3 AGTCGCTCTTAAAAGCCGAG GAGGCTAGCAAATGGAGTCC 50.0 55.0 58.0 58.0 754-781 bp 1.0 83.9
trnK-rbcL_p4 GAGTCGCTCTTAAAAGCCGA GAGGCTAGCAAATGGAGTCC 50.0 55.0 58.0 58.0 755-782 bp 1.0 83.9
trnK-rbcL_p5 TGGAGTCGCTCTTAAAAGCC AGTCCTGAATTAGCTGCTGC 50.0 50.0 57.9 58.0 742-769 bp 1.0 83.3