Hotspot

trnH-psbA

LSC · 135-392 · 258 bp

Back to Millettia

Pi 0.031
MarkerSeek score 67.00
Primer available yes
Label trnH-psbA

Feature Metadata

label_name
trnH-psbA
feature_type
igs
region
LSC
length_bp
258
variable_sites
8
pi
0.031
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-psbA_p1 GATCCACTTGGCTACATCCG CCCTCTAGATCTAGCTGCGA 55.0 55.0 58.1 57.8 325-336 bp 1.0 79.4
trnH-psbA_p2 GATCCACTTGGCTACATCCG TCCCTCTAGATCTAGCTGCG 55.0 55.0 58.1 57.8 326-337 bp 1.0 79.4
trnH-psbA_p3 CAATCCACTGCCTTGATCCA CCCTCTAGATCTAGCTGCGA 50.0 55.0 57.9 57.8 339-350 bp 1.0 79.3
trnH-psbA_p4 CAATCCACTGCCTTGATCCA TCCCTCTAGATCTAGCTGCG 50.0 55.0 57.9 57.8 340-351 bp 1.0 79.2
trnH-psbA_p5 AATCCACTGCCTTGATCCAC CCCTCTAGATCTAGCTGCGA 50.0 55.0 57.9 57.8 338-349 bp 1.0 79.2