Hotspot

ycf1

SSC · 129697-135339 · 5643 bp

Back to Milicia

Pi 0.000709

MarkerSeek score 52.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5643
variable_sites: 4
pi: 0.000709
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCGTGATTTCTTTCATTATTGT	AAAGGATTTATAAGCCCACA	27.3	35.0	52.1	52.1	305 bp	1.0	40.3
ycf1_p2	TCGTGATTTCTTTCATTATTGT	AAAAGGATTTATAAGCCCACA	27.3	33.3	52.1	53.0	306 bp	1.0	40.3
ycf1_p3	TTCGTGATTTCTTTCATTATTGT	AAAGGATTTATAAGCCCACA	26.1	35.0	53.0	52.1	306 bp	1.0	40.3
ycf1_p4	TCGTGATTTCTTTCATTATTGT	AAAAAGGATTTATAAGCCCACA	27.3	31.8	52.1	53.9	307 bp	1.0	40.3
ycf1_p5	TTCGTGATTTCTTTCATTATTGT	AAAAGGATTTATAAGCCCACA	26.1	33.3	53.0	53.0	307 bp	1.0	40.3