Hotspot

rpoA

LSC · 82579-83550 · 972 bp

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Pi 0.00309

MarkerSeek score 60.50

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 972
variable_sites: 3
pi: 0.00309
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	TCGCCTTAAAGTGAATTTTCCC	CGAGACGTAACTCCTATGCC	40.9	55.0	57.2	57.6	1206 bp	1.0	54.8
rpoA_p2	CGCCTTAAAGTGAATTTTCCCT	CGAGACGTAACTCCTATGCC	40.9	55.0	57.2	57.6	1205 bp	1.0	54.7
rpoA_p3	CGCCTTAAAGTGAATTTTCCC	CGAGACGTAACTCCTATGCC	42.9	55.0	55.8	57.6	1205 bp	1.0	51.0
rpoA_p4	TCGCCTTAAAGTGAATTTTCCC	GACGTAACTCCTATGCCACA	40.9	50.0	57.2	57.0	1203 bp	1.0	50.4
rpoA_p5	CGCCTTAAAGTGAATTTTCCCT	GACGTAACTCCTATGCCACA	40.9	50.0	57.2	57.0	1202 bp	1.0	50.3