Hotspot

matK

LSC · 2186-3697 · 1512 bp

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Pi 0.00132

MarkerSeek score 59.50

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1512
variable_sites: 2
pi: 0.00132
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	AGTTGATCAACCCTTACTGCA	AGCCCATCCTATTTTCGGTTC	42.9	47.6	57.2	58.1	1661 bp	1.0	57.2
matK_p2	AGTTGATCAACCCTTACTGCA	AAGCCCATCCTATTTTCGGT	42.9	45.0	57.2	56.9	1662 bp	1.0	56.8
matK_p3	AGTTGATCAACCCTTACTGCA	AGCCCATCCTATTTTCGGTT	42.9	45.0	57.2	56.9	1661 bp	1.0	56.8
matK_p4	AGTTGATCAACCCTTACTGCA	AACCCAATAAAGCCCATCCT	42.9	45.0	57.2	56.8	1671 bp	1.0	55.8
matK_p5	AGTTGATCAACCCTTACTGCA	GCCCATCCTATTTTCGGTTCA	42.9	47.6	57.2	58.3	1660 bp	1.0	55.0