Hotspot

ccsA

SSC · 120064-121029 · 966 bp

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Pi 0

MarkerSeek score 32.40

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 966
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 32.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GCGGCATGACATCTCCTAAA	GTTCTCGCTGGATTACACGA	50.0	50.0	58.0	57.7	1168 bp	1.0	77.4
ccsA_p2	GCGGCATGACATCTCCTAAA	TCTCGCTGGATTACACGAAG	50.0	50.0	58.0	57.4	1166 bp	1.0	75.1
ccsA_p3	GCGGCATGACATCTCCTAAA	TTCAAAAGGTTCTCGCTGGA	50.0	45.0	58.0	57.4	1176 bp	1.0	74.6
ccsA_p4	GCGGCATGACATCTCCTAAA	AGGTTCTCGCTGGATTACAC	50.0	50.0	58.0	57.3	1170 bp	1.0	74.2
ccsA_p5	GCGGCATGACATCTCCTAAA	GGTTCTCGCTGGATTACACG	50.0	55.0	58.0	58.7	1169 bp	1.0	73.9