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ccsA-ndhD

SSC · 121030-121314 · 285 bp

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Pi 0.0351

MarkerSeek score 72.70

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 285
variable_sites: 10
pi: 0.0351
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TTCCGCAATTGTAGCCTCAA	TCAGTTGACAAAGTCGAAGCT	45.0	42.9	57.8	57.8	448 bp	1.0	70.8
ccsA-ndhD_p2	ATTCCGCAATTGTAGCCTCA	TCAGTTGACAAAGTCGAAGCT	45.0	42.9	57.6	57.8	449 bp	1.0	69.0
ccsA-ndhD_p3	TCCGCAATTGTAGCCTCAAT	TCAGTTGACAAAGTCGAAGCT	45.0	42.9	57.6	57.8	447 bp	1.0	69.0
ccsA-ndhD_p4	TTCCGCAATTGTAGCCTCAA	AGTTGACAAAGTCGAAGCTCT	45.0	42.9	57.8	57.5	446 bp	1.0	68.6
ccsA-ndhD_p5	CGCAATTGTAGCCTCAATGG	TCAGTTGACAAAGTCGAAGCT	50.0	42.9	57.5	57.8	445 bp	1.0	68.5