Hotspot

rpl32-trnL-UAG

SSC · 118271-119876 · 1606 bp

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Pi 0.00125
MarkerSeek score 59.20
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1606
variable_sites
2
pi
0.00125
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGGCAGCATTGAAAGCTTTT AGCGTGTCTACCGATTTCAC 45.0 50.0 57.8 58.0 1718-1753 bp 1.0 78.6
rpl32-trnL-UAG_p2 GGGCAGCATTGAAAGCTTTT CAGCGTGTCTACCGATTTCA 45.0 50.0 57.8 58.0 1719-1754 bp 1.0 78.6
rpl32-trnL-UAG_p3 GGGCAGCATTGAAAGCTTTT TTTAGGAGATGTCATGCCGC 45.0 50.0 57.8 58.0 1783-1818 bp 1.0 78.3
rpl32-trnL-UAG_p4 GGGCAGCATTGAAAGCTTTT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.8 57.9 1728-1763 bp 1.0 77.7
rpl32-trnL-UAG_p5 GGGCAGCATTGAAAGCTTTT CTAAGAGCAGCGTGTCTACC 45.0 55.0 57.8 57.8 1726-1761 bp 1.0 77.1