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psbT-psbN

LSC · 78869-78936 · 68 bp

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Pi 0.0147

MarkerSeek score 61.00

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 68
variable_sites: 1
pi: 0.0147
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	TCGAGAACCGCCTAAAGTTC	ACGCTCGTTGCCATCTTTAT	50.0	45.0	57.6	58.0	228 bp	1.0	77.3
psbT-psbN_p2	GAGAACCGCCTAAAGTTCCA	ACGCTCGTTGCCATCTTTAT	50.0	45.0	57.5	58.0	226 bp	1.0	76.4
psbT-psbN_p3	TCGAGAACCGCCTAAAGTTC	TTCGAGGAACACGGAGACTA	50.0	50.0	57.6	57.8	129 bp	1.0	76.2
psbT-psbN_p4	TCGAGAACCGCCTAAAGTTC	TCGAGGAACACGGAGACTAG	50.0	55.0	57.6	58.3	128 bp	1.0	75.6
psbT-psbN_p5	GAGAACCGCCTAAAGTTCCA	TTCGAGGAACACGGAGACTA	50.0	50.0	57.5	57.8	127 bp	1.0	75.4