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rpl20-rps12

LSC · 73317-74125 · 809 bp

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Pi 0.00124

MarkerSeek score 59.60

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 809
variable_sites: 1
pi: 0.00124
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	GAGCCCCTTGAAAGCTTGAT	AAATGTCACGAAATCCCCCG	50.0	50.0	58.2	58.5	957-958 bp	1.0	74.6
rpl20-rps12_p2	GTAAGTCTTGAACGAGCCCC	AAATGTCACGAAATCCCCCG	55.0	50.0	58.3	58.5	970-971 bp	1.0	73.6
rpl20-rps12_p3	TTGGTTCTACGTCTTCGAGC	AAATGTCACGAAATCCCCCG	50.0	50.0	57.7	58.5	922-923 bp	1.0	73.1
rpl20-rps12_p4	GAGCCCCTTGAAAGCTTGAT	CAGCGACGAGGAACATGTAC	50.0	55.0	58.2	58.7	920-921 bp	1.0	73.1
rpl20-rps12_p5	TAAGTCTTGAACGAGCCCCT	AAATGTCACGAAATCCCCCG	50.0	50.0	58.4	58.5	969-970 bp	1.0	73.0