Hotspot

ycf4-cemA

LSC · 64546-65481 · 936 bp

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Pi 0

MarkerSeek score 60.00

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 936
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	CAGAAAGCTGCTGAATTGGC	AGAGAGATCCACCAAGGCAA	50.0	50.0	58.0	58.3	1064-1069 bp	1.0	78.1
ycf4-cemA_p2	CATTCCCTTGACTCGTACCG	AGAGAGATCCACCAAGGCAA	55.0	50.0	58.1	58.3	1113-1118 bp	1.0	77.5
ycf4-cemA_p3	TTGGCCTATTTCTTGCGTGT	AGAGAGATCCACCAAGGCAA	45.0	50.0	58.1	58.3	1049-1054 bp	1.0	77.4
ycf4-cemA_p4	ATTCCCTTGACTCGTACCGA	AGAGAGATCCACCAAGGCAA	50.0	50.0	58.2	58.3	1112-1117 bp	1.0	76.9
ycf4-cemA_p5	TTCCCTTGACTCGTACCGAT	AGAGAGATCCACCAAGGCAA	50.0	50.0	58.2	58.3	1111-1116 bp	1.0	76.9