Hotspot

atpB-rbcL

LSC · 58104-58829 · 726 bp

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Pi 0

MarkerSeek score 59.60

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 726
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	GGAACCCCAGGACTAGAAGT	CACCAGCTTTGAATCCAACAC	55.0	47.6	58.0	58.0	808-813 bp	1.0	72.1
atpB-rbcL_p2	CATCCAGTACCGGACCAATG	CACCAGCTTTGAATCCAACAC	55.0	47.6	58.0	58.0	867-872 bp	1.0	72.0
atpB-rbcL_p3	GGAACCCCAGGACTAGAAGT	ACCAGCTTTGAATCCAACACT	55.0	42.9	58.0	58.1	807-812 bp	1.0	71.8
atpB-rbcL_p4	CATCCAGTACCGGACCAATG	ACCAGCTTTGAATCCAACACT	55.0	42.9	58.0	58.1	866-871 bp	1.0	71.8
atpB-rbcL_p5	TCCAGTACCGGACCAATGAT	CACCAGCTTTGAATCCAACAC	50.0	47.6	58.1	58.0	865-870 bp	1.0	71.4