Pi
0
MarkerSeek score
59.80
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | CGATGACCCTCGCATTACAA | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.1 | 58.0 | 1199-1211 bp | 1.0 | 80.2 |
| trnT-UGU-trnL-UAA_p2 | CGATGACCCTCGCATTACAA | TAGCGTCTACCAATTTCGCC | 50.0 | 50.0 | 58.1 | 58.1 | 1207-1219 bp | 1.0 | 79.7 |
| trnT-UGU-trnL-UAA_p3 | GCGATGCTCTAACCTCTGAG | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 57.9 | 58.0 | 1177-1189 bp | 1.0 | 79.7 |
| trnT-UGU-trnL-UAA_p4 | GCGATGCTCTAACCTCTGAG | TAGCGTCTACCAATTTCGCC | 55.0 | 50.0 | 57.9 | 58.1 | 1185-1197 bp | 1.0 | 79.2 |
| trnT-UGU-trnL-UAA_p5 | CGATGACCCTCGCATTACAA | AGTCCGTAGCGTCTACCAAT | 50.0 | 50.0 | 58.1 | 58.2 | 1213-1225 bp | 1.0 | 78.3 |