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psbZ-trnG-GCC

LSC · 38399-39398 · 1000 bp

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Pi 0.00412
MarkerSeek score 63.90
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
1000
variable_sites
4
pi
0.00412
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 CTTCTCCTGATGGTTGGTCG AATCGAACCCGCATCTTCTC 55.0 50.0 58.0 58.1 1137-1165 bp 1.0 81.7
psbZ-trnG-GCC_p2 CTTCTCCTGATGGTTGGTCG GAATCGAACCCGCATCTTCT 55.0 50.0 58.0 58.1 1138-1166 bp 1.0 81.7
psbZ-trnG-GCC_p3 TTGCTTCTCCTGATGGTTGG AATCGAACCCGCATCTTCTC 50.0 50.0 57.8 58.1 1140-1168 bp 1.0 80.2
psbZ-trnG-GCC_p4 TTGCTTCTCCTGATGGTTGG GAATCGAACCCGCATCTTCT 50.0 50.0 57.8 58.1 1141-1169 bp 1.0 80.2
psbZ-trnG-GCC_p5 GCTTCTCCTGATGGTTGGTC AATCGAACCCGCATCTTCTC 55.0 50.0 58.3 58.1 1138-1166 bp 1.0 79.8