Hotspot

trnC-GCA-petN

LSC · 29660-30675 · 1016 bp

Back to Milicia

Pi 0.000984

MarkerSeek score 59.30

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 1016
variable_sites: 1
pi: 0.000984
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	1082-1107 bp	1.0	78.2
trnC-GCA-petN_p2	GGGTGTCGCCTAATCAACAA	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.2	58.2	1064-1089 bp	1.0	77.4
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.1	57.6	1143-1168 bp	1.0	76.6
trnC-GCA-petN_p4	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	1100-1125 bp	1.0	76.0
trnC-GCA-petN_p5	GGGTGTCGCCTAATCAACAA	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.2	57.6	1125-1150 bp	1.0	75.8