Hotspot

ycf1

SSC · 108180-113258 · 5079 bp

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Pi 0.00663

MarkerSeek score 52.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5079
variable_sites: 173
pi: 0.00663
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCGATCTACAGGTTTGACAA	CAAATTTTTGGGGGTGATTCTC	40.0	40.9	54.0	56.1	104-174 bp	0.875	47.0
ycf1_p2	TCGATCTACAGGTTTGACAAA	CAAATTTTTGGGGGTGATTCTC	38.1	40.9	54.9	56.1	104-174 bp	0.875	47.0
ycf1_p3	ATCGATCTACAGGTTTGACA	CAAATTTTTGGGGGTGATTCTC	40.0	40.9	53.8	56.1	105-175 bp	0.875	47.0
ycf1_p4	TCGATCTACAGGTTTGACAA	CAAATTTTTGGGGGTGATTCT	40.0	38.1	54.0	54.9	103-174 bp	0.875	47.0
ycf1_p5	ATCGATCTACAGGTTTGACAA	CAAATTTTTGGGGGTGATTCTC	38.1	40.9	54.7	56.1	105-175 bp	0.875	47.0