Hotspot

rpl16

LSC · 80881-82306 · 1426 bp

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Pi 0.00441

MarkerSeek score 45.20

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1426
variable_sites: 33
pi: 0.00441
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	ACACATCAATTCTCGAGCCC	ATCTTCTAGCGTCGAGGGAA	50.0	50.0	58.0	57.9	2245-2266 bp	1.0	82.8
rpl16_p2	ACACATCAATTCTCGAGCCC	TCTTCTAGCGTCGAGGGAAT	50.0	50.0	58.0	57.9	2244-2265 bp	1.0	82.8
rpl16_p3	CGCACCAAAAACTCGAGTTC	ATCTTCTAGCGTCGAGGGAA	50.0	50.0	58.0	57.9	2485-2506 bp	1.0	82.6
rpl16_p4	CGCACCAAAAACTCGAGTTC	TCTTCTAGCGTCGAGGGAAT	50.0	50.0	58.0	57.9	2484-2505 bp	1.0	82.6
rpl16_p5	CGCACCAAAAACTCGAGTTC	GTCGAGGGAATTGCACGTAT	50.0	50.0	58.0	58.1	2475-2496 bp	1.0	82.5