Hotspot

rbcL

LSC · 54805-56262 · 1458 bp

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Pi 0.00718

MarkerSeek score 53.20

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1458
variable_sites: 34
pi: 0.00718
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	TCATGAGTTGTAGGGAGGGA	TGGGCCGAGTTTCATTGAAA	50.0	45.0	57.1	58.0	1521-1930 bp	1.0	74.1
rbcL_p2	TCATGAGTTGTAGGGAGGGA	TTGGGCCGAGTTTCATTGAA	50.0	45.0	57.1	58.0	1522-1931 bp	1.0	74.1
rbcL_p3	ACGCCTAATTCATGTCGAGT	TGGGCCGAGTTTCATTGAAA	45.0	45.0	57.0	58.0	1569-1929 bp	1.0	73.9
rbcL_p4	ACGCCTAATTCATGTCGAGT	TTGGGCCGAGTTTCATTGAA	45.0	45.0	57.0	58.0	1570-1930 bp	1.0	73.9
rbcL_p5	TCATGAGTTGTAGGGAGGGA	ATTGGGCCGAGTTTCATTGA	50.0	45.0	57.1	57.8	1523-1932 bp	1.0	72.5