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rrn5-trnR-ACG

SSC · 106955-107241 · 287 bp

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Pi 0.015

MarkerSeek score 65.40

Primer available yes

Label rrn5-trnR-ACG

Feature Metadata

label_name: rrn5-trnR-ACG
feature_type: igs
region: SSC
length_bp: 287
variable_sites: 20
pi: 0.015
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn5-trnR-ACG_p1	GGTGACGATACTGTAGGGGA	CCACGTGCTCTAATCCTCTG	55.0	55.0	57.9	57.8	244-371 bp	1.0	86.3
rrn5-trnR-ACG_p2	AATCCATCCCGAACTTGGTG	CCACGTGCTCTAATCCTCTG	50.0	55.0	57.9	57.8	279-406 bp	1.0	85.4
rrn5-trnR-ACG_p3	CAATCCATCCCGAACTTGGT	CCACGTGCTCTAATCCTCTG	50.0	55.0	57.9	57.8	280-407 bp	1.0	85.4
rrn5-trnR-ACG_p4	ACCAATCCATCCCGAACTTG	CCACGTGCTCTAATCCTCTG	50.0	55.0	57.9	57.8	282-409 bp	1.0	85.3
rrn5-trnR-ACG_p5	CACCAATCCATCCCGAACTT	CCACGTGCTCTAATCCTCTG	50.0	55.0	57.9	57.8	283-410 bp	1.0	85.3