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rpl16-rps3

LSC · 82307-82458 · 152 bp

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Pi 0.0314

MarkerSeek score 66.70

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 152
variable_sites: 18
pi: 0.0314
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	AAATAAAGTTTTCGCGGGCG	AGTAATGGGGGAACATGGGA	45.0	50.0	58.0	58.0	1478-1497 bp	1.0	83.0
rpl16-rps3_p2	AAATAAAGTTTTCGCGGGCG	ATCTTCTAGCGTCGAGGGAA	45.0	50.0	58.0	57.9	1306-1325 bp	1.0	82.9
rpl16-rps3_p3	AAATAAAGTTTTCGCGGGCG	TCTTCTAGCGTCGAGGGAAT	45.0	50.0	58.0	57.9	1305-1324 bp	1.0	82.9
rpl16-rps3_p4	AAATAAAGTTTTCGCGGGCG	GTCGAGGGAATTGCACGTAT	45.0	50.0	58.0	58.1	1296-1315 bp	1.0	82.8
rpl16-rps3_p5	AAATAAAGTTTTCGCGGGCG	GTAATGGGGGAACATGGGAC	45.0	55.0	58.0	57.9	1477-1496 bp	1.0	82.5