Hotspot

rpl36-infA

LSC · 79339-79453 · 115 bp

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Pi 0.0189
MarkerSeek score 69.50
Primer available yes
Label rpl36-infA

Feature Metadata

label_name
rpl36-infA
feature_type
igs
region
LSC
length_bp
115
variable_sites
10
pi
0.0189
unique_haplotype_count
11
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl36-infA_p1 GTTTATGTCTTGGGTTGGAACA ACTCCGCAACAAAGATTCGA 40.9 45.0 56.8 57.8 216-243 bp 1.0 60.5
rpl36-infA_p2 GTTTATGTCTTGGGTTGGAACA TCGACTCCGCAACAAAGATT 40.9 45.0 56.8 57.8 219-246 bp 1.0 60.4
rpl36-infA_p3 TGTTTATGTCTTGGGTTGGAAC ACTCCGCAACAAAGATTCGA 40.9 45.0 56.8 57.8 217-244 bp 1.0 60.4
rpl36-infA_p4 TGTTTATGTCTTGGGTTGGAAC TCGACTCCGCAACAAAGATT 40.9 45.0 56.8 57.8 220-247 bp 1.0 60.4
rpl36-infA_p5 GTTTATGTCTTGGGTTGGAACA ATCGACTCCGCAACAAAGAT 40.9 45.0 56.8 57.6 220-247 bp 1.0 58.6