Hotspot

psaI-ycf4

LSC · 59092-59494 · 403 bp

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Pi 0.0111

MarkerSeek score 65.40

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 403
variable_sites: 21
pi: 0.0111
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	GCAATCGCAATGGCTTCTTT	TCGAGCCCCCTTTATAGGTT	45.0	50.0	58.0	57.8	497-524 bp	1.0	84.8
psaI-ycf4_p2	GCAATCGCAATGGCTTCTTT	TTCGAGCCCCCTTTATAGGT	45.0	50.0	58.0	57.8	498-525 bp	1.0	84.8
psaI-ycf4_p3	GCAATCGCAATGGCTTCTTT	TACTTGTTTTTCGAGCCCCC	45.0	50.0	58.0	58.1	507-534 bp	0.9375	82.7
psaI-ycf4_p4	TAGGCCTAGTATTTCCGGCA	TACTTGTTTTTCGAGCCCCC	50.0	50.0	57.9	58.1	524-551 bp	0.9375	82.0
psaI-ycf4_p5	GCAATCGCAATGGCTTCTTT	TTGTTTTTCGAGCCCCCTTT	45.0	45.0	58.0	58.2	504-531 bp	0.9375	81.8