Hotspot

atpB-rbcL

LSC · 54013-54804 · 792 bp

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Pi 0.00644

MarkerSeek score 67.80

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 792
variable_sites: 29
pi: 0.00644
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CTGGCGGAAAAGCTACATCT	CACCAGCTTTGAATCCAACAC	50.0	47.6	58.0	58.0	500-953 bp	1.0	78.5
atpB-rbcL_p2	CTGGCGGAAAAGCTACATCT	ACCAGCTTTGAATCCAACACT	50.0	42.9	58.0	58.1	501-952 bp	1.0	78.2
atpB-rbcL_p3	GGCAACCCCAGAACTAGAAG	CACCAGCTTTGAATCCAACAC	55.0	47.6	57.9	58.0	427-880 bp	1.0	78.0
atpB-rbcL_p4	GATACGCCCCAGGTTCTTTT	CACCAGCTTTGAATCCAACAC	50.0	47.6	57.9	58.0	457-910 bp	1.0	77.8
atpB-rbcL_p5	GGCAACCCCAGAACTAGAAG	ACCAGCTTTGAATCCAACACT	55.0	42.9	57.9	58.1	428-879 bp	1.0	77.7