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atpI-atpH

LSC · 25216-26383 · 1168 bp

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Pi 0.00927

MarkerSeek score 71.00

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 1168
variable_sites: 48
pi: 0.00927
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	CCCGTCATGTTCCTTGGATT	ATAACGGAAGCGGCAGAAAT	50.0	45.0	57.9	58.0	1288-1307 bp	1.0	83.4
atpI-atpH_p2	CCCGTCATGTTCCTTGGATT	TACCTTGACCAACTCCAGGT	50.0	50.0	57.9	57.9	1344-1363 bp	1.0	82.6
atpI-atpH_p3	CCCGTCATGTTCCTTGGATT	AGCCAATCCAGCAGCAATAA	50.0	45.0	57.9	57.9	1304-1323 bp	1.0	82.5
atpI-atpH_p4	CCCGTCATGTTCCTTGGATT	AATAACGGAAGCGGCAGAAA	50.0	45.0	57.9	58.2	1289-1308 bp	1.0	82.2
atpI-atpH_p5	CCCGTCATGTTCCTTGGATT	GCAGTACCTTGACCAACTCC	50.0	55.0	57.9	58.2	1348-1367 bp	1.0	82.0