primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	CCGTGCTAACCTTGGTATGG	20	20	55.0	55.0	58.1	58.3	0.0	0.0	0.465225	74	461	505-526	1.000000	83.0
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.469587	74	461	519-540	1.000000	82.8
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTGCCTTGATCCAC	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.471094	74	461	518-539	1.000000	82.8
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCCACTGCCTTGATCC	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.471094	74	461	520-541	1.000000	82.8
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	GATCCACTTGGCTACATCCG	ACCGTGCTAACCTTGGTATG	20	20	55.0	50.0	58.1	57.6	0.0	0.0	0.525510	74	461	506-527	1.000000	82.5
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AAAGCCGAGTACTCTACCGT	TTCCTTGAAAAAGGCGCTCA	20	20	50.0	45.0	58.2	58.3	20.3	7.6	0.482065	4382	5200	946-974	1.000000	80.9
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAGCCGAGTACTCTACCGTT	TTCCTTGAAAAAGGCGCTCA	20	20	50.0	45.0	58.2	58.3	20.3	7.6	0.482065	4382	5200	945-973	1.000000	80.9
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	CCGCACTTAAAAGCCGAGTA	TTCCTTGAAAAAGGCGCTCA	20	20	50.0	45.0	58.3	58.3	5.3	7.6	0.596451	4382	5200	955-983	1.000000	80.1
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	TACCGTTGAGTTAGCAACCC	TTCCTTGAAAAAGGCGCTCA	20	20	50.0	45.0	57.5	58.3	0.0	7.6	0.774402	4382	5200	932-960	1.000000	78.7
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AAGCCGAGTACTCTACCGTT	AGGCAGAGGTTTTTAAGGCA	20	20	50.0	45.0	58.2	57.3	20.3	0.0	0.844077	4382	5200	893-921	1.000000	78.2
atpI-atpH_p1	atpI-atpH	1	CCCGTCATGTTCCTTGGATT	ATAACGGAAGCGGCAGAAAT	20	20	50.0	45.0	57.9	58.0	0.0	1.4	0.167157	25216	26383	1288-1307	1.000000	83.4
atpI-atpH_p2	atpI-atpH	2	CCCGTCATGTTCCTTGGATT	TACCTTGACCAACTCCAGGT	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.241050	25216	26383	1344-1363	1.000000	82.6
atpI-atpH_p3	atpI-atpH	3	CCCGTCATGTTCCTTGGATT	AGCCAATCCAGCAGCAATAA	20	20	50.0	45.0	57.9	57.9	0.0	0.0	0.277216	25216	26383	1304-1323	1.000000	82.5
atpI-atpH_p4	atpI-atpH	4	CCCGTCATGTTCCTTGGATT	AATAACGGAAGCGGCAGAAA	20	20	50.0	45.0	57.9	58.2	0.0	1.4	0.312696	25216	26383	1289-1308	1.000000	82.2
atpI-atpH_p5	atpI-atpH	5	CCCGTCATGTTCCTTGGATT	GCAGTACCTTGACCAACTCC	20	20	50.0	55.0	57.9	58.2	0.0	0.0	0.319087	25216	26383	1348-1367	1.000000	82.0
ycf3-trnS-GGA_p1	ycf3-trnS-GGA	1	TTCTCCTGAAGTTGTCGGAA	CAACCACTCAGCCATCTCTC	20	20	45.0	55.0	56.4	58.0	0.0	0.0	1.605526	44074	44988	805-1051	1.000000	73.4
ycf3-trnS-GGA_p2	ycf3-trnS-GGA	2	TTTCTCCTGAAGTTGTCGGA	CAACCACTCAGCCATCTCTC	20	20	45.0	55.0	56.4	58.0	0.0	0.0	1.605526	44074	44988	806-1052	1.000000	73.4
ycf3-trnS-GGA_p3	ycf3-trnS-GGA	3	TTCTCCTGAAGTTGTCGGAA	ATGCTACGCTTTCAACCACT	20	20	45.0	45.0	56.4	57.8	0.0	0.0	1.769175	44074	44988	817-1063	1.000000	72.1
ycf3-trnS-GGA_p4	ycf3-trnS-GGA	4	TTTCTCCTGAAGTTGTCGGA	ATGCTACGCTTTCAACCACT	20	20	45.0	45.0	56.4	57.8	0.0	0.0	1.769175	44074	44988	818-1064	1.000000	72.1
ycf3-trnS-GGA_p5	ycf3-trnS-GGA	5	TTCTCCTGAAGTTGTCGGAA	GGATTCGAACCCTCGGTAAG	20	20	45.0	55.0	56.4	57.8	0.0	0.0	1.804650	44074	44988	860-1106	1.000000	71.7
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	TCCTTTGCTACTCGGAGCTA	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.9	57.8	4.6	0.0	0.336606	49846	51012	1275-1359	1.000000	83.8
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	ACTCGGAGCTAAAAATCCCG	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.7	57.8	4.6	0.0	0.508407	49846	51012	1266-1350	1.000000	82.4
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	TTCTCCGGTCCTTTGCTACT	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	58.4	57.8	0.0	0.0	0.556367	49846	51012	1283-1367	1.000000	82.0
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	TTTCTCCGGTCCTTTGCTAC	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.5	57.8	0.0	0.0	0.664849	49846	51012	1284-1368	1.000000	81.1
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	TCCTTTGCTACTCGGAGCTA	GAAGGTCTACGGTTCGAGTC	20	20	50.0	55.0	57.9	57.5	4.6	0.0	0.680379	49846	51012	1274-1358	1.000000	81.0
atpB-rbcL_p1	atpB-rbcL	1	CTGGCGGAAAAGCTACATCT	CACCAGCTTTGAATCCAACAC	20	21	50.0	47.6	58.0	58.0	0.0	0.0	1.047103	54013	54804	500-953	1.000000	78.5
atpB-rbcL_p2	atpB-rbcL	2	CTGGCGGAAAAGCTACATCT	ACCAGCTTTGAATCCAACACT	20	21	50.0	42.9	58.0	58.1	0.0	0.0	1.080391	54013	54804	501-952	1.000000	78.2
atpB-rbcL_p3	atpB-rbcL	3	GGCAACCCCAGAACTAGAAG	CACCAGCTTTGAATCCAACAC	20	21	55.0	47.6	57.9	58.0	0.0	0.0	1.132608	54013	54804	427-880	1.000000	78.0
atpB-rbcL_p4	atpB-rbcL	4	GATACGCCCCAGGTTCTTTT	CACCAGCTTTGAATCCAACAC	20	21	50.0	47.6	57.9	58.0	0.0	0.0	1.141542	54013	54804	457-910	1.000000	77.8
atpB-rbcL_p5	atpB-rbcL	5	GGCAACCCCAGAACTAGAAG	ACCAGCTTTGAATCCAACACT	20	21	55.0	42.9	57.9	58.1	0.0	0.0	1.165895	54013	54804	428-879	1.000000	77.7
rbcL_p1	rbcL	1	TCATGAGTTGTAGGGAGGGA	TGGGCCGAGTTTCATTGAAA	20	20	50.0	45.0	57.1	58.0	0.0	7.7	0.952221	54805	56262	1521-1930	1.000000	74.1
rbcL_p2	rbcL	2	TCATGAGTTGTAGGGAGGGA	TTGGGCCGAGTTTCATTGAA	20	20	50.0	45.0	57.1	58.0	0.0	0.0	0.952221	54805	56262	1522-1931	1.000000	74.1
rbcL_p3	rbcL	3	ACGCCTAATTCATGTCGAGT	TGGGCCGAGTTTCATTGAAA	20	20	45.0	45.0	57.0	58.0	0.0	7.7	0.973233	54805	56262	1569-1929	1.000000	73.9
rbcL_p4	rbcL	4	ACGCCTAATTCATGTCGAGT	TTGGGCCGAGTTTCATTGAA	20	20	45.0	45.0	57.0	58.0	0.0	0.0	0.973233	54805	56262	1570-1930	1.000000	73.9
rbcL_p5	rbcL	5	TCATGAGTTGTAGGGAGGGA	ATTGGGCCGAGTTTCATTGA	20	20	50.0	45.0	57.1	57.8	0.0	0.0	1.154890	54805	56262	1523-1932	1.000000	72.5
psaI-ycf4_p1	psaI-ycf4	1	GCAATCGCAATGGCTTCTTT	TCGAGCCCCCTTTATAGGTT	20	20	45.0	50.0	58.0	57.8	0.0	0.0	0.186092	59092	59494	497-524	1.000000	84.8
psaI-ycf4_p2	psaI-ycf4	2	GCAATCGCAATGGCTTCTTT	TTCGAGCCCCCTTTATAGGT	20	20	45.0	50.0	58.0	57.8	0.0	0.0	0.186092	59092	59494	498-525	1.000000	84.8
psaI-ycf4_p3	psaI-ycf4	3	GCAATCGCAATGGCTTCTTT	TACTTGTTTTTCGAGCCCCC	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.100184	59092	59494	507-534	0.937500	82.7
psaI-ycf4_p4	psaI-ycf4	4	TAGGCCTAGTATTTCCGGCA	TACTTGTTTTTCGAGCCCCC	20	20	50.0	50.0	57.9	58.1	8.9	0.0	0.190853	59092	59494	524-551	0.937500	82.0
psaI-ycf4_p5	psaI-ycf4	5	GCAATCGCAATGGCTTCTTT	TTGTTTTTCGAGCCCCCTTT	20	20	45.0	45.0	58.0	58.2	0.0	0.0	0.217358	59092	59494	504-531	0.937500	81.8
petA-psbJ_p1	petA-psbJ	1	ACGTGTACAAGGCCTTTTGT	ATTCCGGATTGGGTTCATCC	20	20	45.0	50.0	58.0	57.6	17.6	4.1	0.411974	62791	63568	915-939	1.000000	79.9
petA-psbJ_p2	petA-psbJ	2	GGCCTTTTGTTCTTCTTCGC	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	57.9	57.6	0.0	4.1	0.416851	62791	63568	905-929	1.000000	79.9
petA-psbJ_p3	petA-psbJ	3	ACGTGTACAAGGCCTTTTGT	TGTGATCGGTTTAGTAGGCG	20	20	45.0	50.0	58.0	57.4	17.6	0.0	0.619192	62791	63568	953-977	1.000000	78.1
petA-psbJ_p4	petA-psbJ	4	ACGTGTACAAGGCCTTTTGT	GTGATCGGTTTAGTAGGCGT	20	20	45.0	50.0	58.0	57.4	17.6	0.0	0.620943	62791	63568	952-976	1.000000	78.1
petA-psbJ_p5	petA-psbJ	5	GGCCTTTTGTTCTTCTTCGC	TGTGATCGGTTTAGTAGGCG	20	20	50.0	50.0	57.9	57.4	0.0	0.0	0.624069	62791	63568	943-967	1.000000	78.1
rpl36-infA_p1	rpl36-infA	1	GTTTATGTCTTGGGTTGGAACA	ACTCCGCAACAAAGATTCGA	22	20	40.9	45.0	56.8	57.8	0.0	0.0	3.357830	79339	79453	216-243	1.000000	60.5
rpl36-infA_p2	rpl36-infA	2	GTTTATGTCTTGGGTTGGAACA	TCGACTCCGCAACAAAGATT	22	20	40.9	45.0	56.8	57.8	0.0	0.0	3.357830	79339	79453	219-246	1.000000	60.4
rpl36-infA_p3	rpl36-infA	3	TGTTTATGTCTTGGGTTGGAAC	ACTCCGCAACAAAGATTCGA	22	20	40.9	45.0	56.8	57.8	0.0	0.0	3.357830	79339	79453	217-244	1.000000	60.4
rpl36-infA_p4	rpl36-infA	4	TGTTTATGTCTTGGGTTGGAAC	TCGACTCCGCAACAAAGATT	22	20	40.9	45.0	56.8	57.8	0.0	0.0	3.357830	79339	79453	220-247	1.000000	60.4
rpl36-infA_p5	rpl36-infA	5	GTTTATGTCTTGGGTTGGAACA	ATCGACTCCGCAACAAAGAT	22	20	40.9	45.0	56.8	57.6	0.0	0.0	3.580673	79339	79453	220-247	1.000000	58.6
rpl16_p1	rpl16	1	ACACATCAATTCTCGAGCCC	ATCTTCTAGCGTCGAGGGAA	20	20	50.0	50.0	58.0	57.9	7.8	7.3	0.084070	80881	82306	2245-2266	1.000000	82.8
rpl16_p2	rpl16	2	ACACATCAATTCTCGAGCCC	TCTTCTAGCGTCGAGGGAAT	20	20	50.0	50.0	58.0	57.9	7.8	7.3	0.084070	80881	82306	2244-2265	1.000000	82.8
rpl16_p3	rpl16	3	CGCACCAAAAACTCGAGTTC	ATCTTCTAGCGTCGAGGGAA	20	20	50.0	50.0	58.0	57.9	29.8	7.3	0.072949	80881	82306	2485-2506	1.000000	82.6
rpl16_p4	rpl16	4	CGCACCAAAAACTCGAGTTC	TCTTCTAGCGTCGAGGGAAT	20	20	50.0	50.0	58.0	57.9	29.8	7.3	0.072949	80881	82306	2484-2505	1.000000	82.6
rpl16_p5	rpl16	5	CGCACCAAAAACTCGAGTTC	GTCGAGGGAATTGCACGTAT	20	20	50.0	50.0	58.0	58.1	29.8	13.2	0.082785	80881	82306	2475-2496	1.000000	82.5
rpl16-rps3_p1	rpl16-rps3	1	AAATAAAGTTTTCGCGGGCG	AGTAATGGGGGAACATGGGA	20	20	45.0	50.0	58.0	58.0	6.1	0.0	0.033810	82307	82458	1478-1497	1.000000	83.0
rpl16-rps3_p2	rpl16-rps3	2	AAATAAAGTTTTCGCGGGCG	ATCTTCTAGCGTCGAGGGAA	20	20	45.0	50.0	58.0	57.9	6.1	7.3	0.082440	82307	82458	1306-1325	1.000000	82.9
rpl16-rps3_p3	rpl16-rps3	3	AAATAAAGTTTTCGCGGGCG	TCTTCTAGCGTCGAGGGAAT	20	20	45.0	50.0	58.0	57.9	6.1	7.3	0.082440	82307	82458	1305-1324	1.000000	82.9
rpl16-rps3_p4	rpl16-rps3	4	AAATAAAGTTTTCGCGGGCG	GTCGAGGGAATTGCACGTAT	20	20	45.0	50.0	58.0	58.1	6.1	13.2	0.092276	82307	82458	1296-1315	1.000000	82.8
rpl16-rps3_p5	rpl16-rps3	5	AAATAAAGTTTTCGCGGGCG	GTAATGGGGGAACATGGGAC	20	20	45.0	55.0	58.0	57.9	6.1	0.0	0.095574	82307	82458	1477-1496	1.000000	82.5
rrn5-trnR-ACG_p1	rrn5-trnR-ACG	1	GGTGACGATACTGTAGGGGA	CCACGTGCTCTAATCCTCTG	20	20	55.0	55.0	57.9	57.8	0.0	10.9	0.278878	106955	107241	244-371	1.000000	86.3
rrn5-trnR-ACG_p2	rrn5-trnR-ACG	2	AATCCATCCCGAACTTGGTG	CCACGTGCTCTAATCCTCTG	20	20	50.0	55.0	57.9	57.8	0.0	10.9	0.344678	106955	107241	279-406	1.000000	85.4
rrn5-trnR-ACG_p3	rrn5-trnR-ACG	3	CAATCCATCCCGAACTTGGT	CCACGTGCTCTAATCCTCTG	20	20	50.0	55.0	57.9	57.8	0.0	10.9	0.344678	106955	107241	280-407	1.000000	85.4
rrn5-trnR-ACG_p4	rrn5-trnR-ACG	4	ACCAATCCATCCCGAACTTG	CCACGTGCTCTAATCCTCTG	20	20	50.0	55.0	57.9	57.8	0.0	10.9	0.344678	106955	107241	282-409	1.000000	85.3
rrn5-trnR-ACG_p5	rrn5-trnR-ACG	5	CACCAATCCATCCCGAACTT	CCACGTGCTCTAATCCTCTG	20	20	50.0	55.0	57.9	57.8	0.0	10.9	0.344678	106955	107241	283-410	1.000000	85.3
ycf1_p1	ycf1	1	TCGATCTACAGGTTTGACAA	CAAATTTTTGGGGGTGATTCTC	20	22	40.0	40.9	54.0	56.1	0.0	0.0	7.848595	110980	111099	104-174	0.875000	47.0
ycf1_p2	ycf1	2	TCGATCTACAGGTTTGACAAA	CAAATTTTTGGGGGTGATTCTC	21	22	38.1	40.9	54.9	56.1	0.0	0.0	7.985843	110980	111099	104-174	0.875000	47.0
ycf1_p3	ycf1	3	ATCGATCTACAGGTTTGACA	CAAATTTTTGGGGGTGATTCTC	20	22	40.0	40.9	53.8	56.1	0.0	0.0	8.089835	110980	111099	105-175	0.875000	47.0
ycf1_p4	ycf1	4	TCGATCTACAGGTTTGACAA	CAAATTTTTGGGGGTGATTCT	20	21	40.0	38.1	54.0	54.9	0.0	0.0	8.097983	110980	111099	103-174	0.875000	47.0
ycf1_p5	ycf1	5	ATCGATCTACAGGTTTGACAA	CAAATTTTTGGGGGTGATTCTC	21	22	38.1	40.9	54.7	56.1	0.0	0.0	8.212452	110980	111099	105-175	0.875000	47.0
trnL-UAG-rpl32_p1	trnL-UAG-rpl32	1	AGCGTGTCTACCGATTTCAC	AAAGGAAAGGATATGGGGCG	20	20	50.0	50.0	58.0	57.6	0.0	0.0	0.372803	122858	123601	860-893	1.000000	83.4
trnL-UAG-rpl32_p2	trnL-UAG-rpl32	2	CAGCGTGTCTACCGATTTCA	AAAGGAAAGGATATGGGGCG	20	20	50.0	50.0	58.0	57.6	0.0	0.0	0.374173	122858	123601	861-894	1.000000	83.4
trnL-UAG-rpl32_p3	trnL-UAG-rpl32	3	AGCGTGTCTACCGATTTCAC	CGGCGTTAAAAGCTTTGTCA	20	20	50.0	45.0	58.0	57.6	0.0	13.5	0.400763	122858	123601	842-875	1.000000	83.3
trnL-UAG-rpl32_p4	trnL-UAG-rpl32	4	CAGCGTGTCTACCGATTTCA	CGGCGTTAAAAGCTTTGTCA	20	20	50.0	45.0	58.0	57.6	0.0	13.5	0.402133	122858	123601	843-876	1.000000	83.2
trnL-UAG-rpl32_p5	trnL-UAG-rpl32	5	GAAGTAGCAAAGCCTTGGGT	AAAGGAAAGGATATGGGGCG	20	20	50.0	50.0	58.1	57.6	0.0	0.0	0.453915	122858	123601	1362-1395	1.000000	80.8
rpl32-ndhF_p1	rpl32-ndhF	1	CGCCCCATATCCTTTCCTTT	GTTGCCGTCGGAAAAAGAAG	20	20	50.0	50.0	57.6	58.0	0.0	17.5	0.397386	123767	124774	1109-1211	1.000000	85.0
rpl32-ndhF_p2	rpl32-ndhF	2	CGCCCCATATCCTTTCCTTT	TGCCGTCGGAAAAAGAAGAA	20	20	50.0	45.0	57.6	58.0	0.0	17.5	0.408676	123767	124774	1107-1209	1.000000	85.0
rpl32-ndhF_p3	rpl32-ndhF	3	CGCCCCATATCCTTTCCTTT	TTGCCGTCGGAAAAAGAAGA	20	20	50.0	45.0	57.6	58.0	0.0	17.5	0.408676	123767	124774	1108-1210	1.000000	85.0
rpl32-ndhF_p4	rpl32-ndhF	4	TGACAAAGCTTTTAACGCCG	GTTGCCGTCGGAAAAAGAAG	20	20	45.0	50.0	57.6	58.0	9.5	17.5	0.425347	123767	124774	1127-1229	1.000000	84.8
rpl32-ndhF_p5	rpl32-ndhF	5	TGACAAAGCTTTTAACGCCG	TGCCGTCGGAAAAAGAAGAA	20	20	45.0	45.0	57.6	58.0	9.5	17.5	0.436636	123767	124774	1125-1227	1.000000	84.7
rrn23_p1	rrn23	1	AAACTTCTGTCGCTCCATCC	TCCATCCATTTAGCAGCACC	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.157742	129397	132208	2870-2951	1.000000	86.6
rrn23_p2	rrn23	2	AAACTTCTGTCGCTCCATCC	CCATCCATTTAGCAGCACCT	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.163575	129397	132208	2869-2950	1.000000	86.6
rrn23_p3	rrn23	3	AAACTTCTGTCGCTCCATCC	CGGCGGTTCGATCTATGATT	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.166597	129397	132208	2914-2995	1.000000	86.5
rrn23_p4	rrn23	4	GCCGTGACCTTCTCTTGAAT	TCCATCCATTTAGCAGCACC	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.157742	129397	132208	2895-2976	0.937500	80.8
rrn23_p5	rrn23	5	GCCGTGACCTTCTCTTGAAT	CCATCCATTTAGCAGCACCT	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.163575	129397	132208	2894-2975	0.937500	80.8