Hotspot

ndhF-rpl32

SSC · 108967-109597 · 631 bp

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Pi 0.0281

MarkerSeek score 65.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 631
variable_sites: 17
pi: 0.0281
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TCGGAAAAAGAAGAAGCCCC	CTACAGGGTGAAGCGAGTTC	50.0	55.0	58.1	58.0	989-1016 bp	1.0	83.2
ndhF-rpl32_p2	TCGGAAAAAGAAGAAGCCCC	CTGTCCAATATCCCCTCCCT	50.0	55.0	58.1	57.9	777-804 bp	1.0	82.9
ndhF-rpl32_p3	TCGGAAAAAGAAGAAGCCCC	TGCCGCACAACAAAACTTTT	50.0	40.0	58.1	57.9	844-871 bp	1.0	82.8
ndhF-rpl32_p4	TCGGAAAAAGAAGAAGCCCC	TTGCCGCACAACAAAACTTT	50.0	40.0	58.1	57.9	845-872 bp	1.0	82.8
ndhF-rpl32_p5	TCGGAAAAAGAAGAAGCCCC	TTTGCCGCACAACAAAACTT	50.0	40.0	58.1	57.9	846-873 bp	1.0	82.8