Hotspot

psbJ-psbL

LSC · 61627-61764 · 138 bp

Back to Microula

Pi 0.0376

MarkerSeek score 77.50

Primer available yes

Label psbJ-psbL

Feature Metadata

label_name: psbJ-psbL
feature_type: igs
region: LSC
length_bp: 138
variable_sites: 5
pi: 0.0376
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbJ-psbL_p1	GGATGAACCCAATCCGGAAT	TGACACAATCAAACCCAAACG	50.0	42.9	57.6	57.6	366-371 bp	1.0	67.8
psbJ-psbL_p2	GGATGAACCCAATCCGGAAT	GACACAATCAAACCCAAACGA	50.0	42.9	57.6	57.3	365-370 bp	1.0	65.6
psbJ-psbL_p3	GGATGAACCCAATCCGGAAT	ACACAATCAAACCCAAACGA	50.0	40.0	57.6	56.1	364-369 bp	1.0	63.9
psbJ-psbL_p4	GGATGAACCCAATCCGGAAT	ATGACACAATCAAACCCAAACG	50.0	40.9	57.6	58.0	367-372 bp	1.0	63.1
psbJ-psbL_p5	GGATGAACCCAATCCGGAAT	GACACAATCAAACCCAAACGAA	50.0	40.9	57.6	58.0	365-370 bp	1.0	63.0