Pi
0.0133
MarkerSeek score
62.20
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | AAAATGGGAAAAGCCGGCTA | ACCAATTTCGCCATATCCCC | 45.0 | 50.0 | 58.1 | 58.0 | 785-805 bp | 1.0 | 82.8 |
| trnT-UGU-trnL-UAA_p2 | GCGATGCTCTAACCTCTGAG | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 57.9 | 58.0 | 731-751 bp | 1.0 | 82.6 |
| trnT-UGU-trnL-UAA_p3 | AAAATGGGAAAAGCCGGCTA | TAGCGTCTACCAATTTCGCC | 45.0 | 50.0 | 58.1 | 58.1 | 793-813 bp | 1.0 | 82.3 |
| trnT-UGU-trnL-UAA_p4 | AAATGGGAAAAGCCGGCTAT | ACCAATTTCGCCATATCCCC | 45.0 | 50.0 | 57.8 | 58.0 | 784-804 bp | 1.0 | 82.1 |
| trnT-UGU-trnL-UAA_p5 | GCGATGCTCTAACCTCTGAG | TAGCGTCTACCAATTTCGCC | 55.0 | 50.0 | 57.9 | 58.1 | 739-759 bp | 1.0 | 82.0 |