Hotspot

rps4-trnT-UGU

LSC · 45057-45439 · 383 bp

Back to Microula

Pi 0.0437

MarkerSeek score 81.80

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 383
variable_sites: 16
pi: 0.0437
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	AGGTCCTCGATAACGTGACA	GTAGGTAGTAGGCCCGCTTA	50.0	55.0	58.2	58.0	471-488 bp	1.0	84.0
rps4-trnT-UGU_p2	AGGTCCTCGATAACGTGACA	TATGTAGGTAGTAGGCCCGC	50.0	55.0	58.2	58.1	474-491 bp	1.0	83.4
rps4-trnT-UGU_p3	AGGTCCTCGATAACGTGACA	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.2	57.9	450-467 bp	1.0	83.3
rps4-trnT-UGU_p4	AGATCACTTCCGGCTCTAGG	GTAGGTAGTAGGCCCGCTTA	55.0	55.0	58.3	58.0	545-562 bp	1.0	82.3
rps4-trnT-UGU_p5	AGATCACTTCCGGCTCTAGG	TATGTAGGTAGTAGGCCCGC	55.0	55.0	58.3	58.1	548-565 bp	1.0	81.7