Hotspot

ycf3-trnS-GGA

LSC · 43297-44052 · 756 bp

Back to Microula

Pi 0.0194

MarkerSeek score 70.20

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 756
variable_sites: 14
pi: 0.0194
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	CATTTATCCGCGATCTAGGCA	AAGGTTCGGAAAGAGAGGGA	47.6	50.0	58.0	58.0	859-871 bp	1.0	75.9
ycf3-trnS-GGA_p2	CATTTATCCGCGATCTAGGCA	CCCTCGGTAAACAAAAGCCT	47.6	50.0	58.0	58.1	833-845 bp	1.0	75.5
ycf3-trnS-GGA_p3	CATTTATCCGCGATCTAGGCA	TCGGAAAGAGAGGGATTCGA	47.6	50.0	58.0	57.9	854-866 bp	1.0	75.0
ycf3-trnS-GGA_p4	CATTTATCCGCGATCTAGGCA	TTCCAATGCTACGCCTTGAA	47.6	45.0	58.0	57.8	804-816 bp	1.0	74.9
ycf3-trnS-GGA_p5	CATTTATCCGCGATCTAGGCA	AGGTTCGGAAAGAGAGGGAT	47.6	50.0	58.0	57.7	858-870 bp	1.0	74.1