Hotspot

trnS-GCU-trnG-UCC

LSC · 7938-8695 · 758 bp

Back to Microula

Pi 0.0332
MarkerSeek score 69.30
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
758
variable_sites
24
pi
0.0332
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 GCTTTAGTCCACTCAGCCAT GGGGTAATAGAACGAATCACACT 50.0 43.5 57.6 58.0 829-839 bp 1.0 58.3
trnS-GCU-trnG-UCC_p2 AGTCCACTCAGCCATCTCTC GGGGTAATAGAACGAATCACACT 55.0 43.5 58.5 58.0 824-834 bp 1.0 57.5
trnS-GCU-trnG-UCC_p3 GCTTTAGTCCACTCAGCCAT GGGGTAATAGAACGAATCACAC 50.0 45.5 57.6 56.6 829-839 bp 1.0 55.3
trnS-GCU-trnG-UCC_p4 TAGTCCACTCAGCCATCTCT GGGGTAATAGAACGAATCACACT 50.0 43.5 57.2 58.0 825-835 bp 1.0 54.9
trnS-GCU-trnG-UCC_p5 AGTCCACTCAGCCATCTCTC GGGGTAATAGAACGAATCACAC 55.0 45.5 58.5 56.6 824-834 bp 1.0 54.5