Hotspot

trnH-GUG-psbA

LSC · 99-401 · 303 bp

Back to Microula

Pi 0.0825
MarkerSeek score 85.40
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
303
variable_sites
25
pi
0.0825
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
85.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG CAACCGTGCTAACCTTGGTA 55.0 50.0 58.1 57.8 424-428 bp 1.0 82.3
trnH-GUG-psbA_p2 GATCCACTTGGCTACATCCG ATCAACCGTGCTAACCTTGG 55.0 50.0 58.1 58.2 426-430 bp 1.0 82.2
trnH-GUG-psbA_p3 CAATCCACTGCCTTGATCCA CAACCGTGCTAACCTTGGTA 50.0 50.0 57.9 57.8 438-442 bp 1.0 82.1
trnH-GUG-psbA_p4 AATCCACTGCCTTGATCCAC CAACCGTGCTAACCTTGGTA 50.0 50.0 57.9 57.8 437-441 bp 1.0 82.1
trnH-GUG-psbA_p5 ACAATCCACTGCCTTGATCC CAACCGTGCTAACCTTGGTA 50.0 50.0 57.9 57.8 439-443 bp 1.0 82.1