Hotspot

rpl16

LSC · 85488-86963 · 1476 bp

Back to Microtropis

Pi 0.00692

MarkerSeek score 56.40

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1476
variable_sites: 10
pi: 0.00692
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	AGCTCTTTTCTTTGGGTGGG	TCAACTCTAGCAATTCTATTCGCT	50.0	37.5	58.0	58.1	1580-1598 bp	1.0	49.3
rpl16_p2	AGCTCTTTTCTTTGGGTGGG	TCAACTCTAGCAATTCTATTCGC	50.0	39.1	58.0	56.8	1580-1598 bp	1.0	48.3
rpl16_p3	AGCTCTTTTCTTTGGGTGGG	CAACTCTAGCAATTCTATTCGCT	50.0	39.1	58.0	56.8	1579-1597 bp	1.0	48.2
rpl16_p4	AGCTCTTTTCTTTGGGTGGG	AGGGTTGAATAAAAATGCGATTGA	50.0	33.3	58.0	57.7	1549-1567 bp	1.0	47.9
rpl16_p5	AGCTCTTTTCTTTGGGTGGG	TGCGATTGAGAATTTCATCTTGA	50.0	34.8	58.0	56.9	1552 bp	0.5	26.9