Hotspot

trnM-CAU-atpE

LSC · 56427-56591 · 165 bp

Back to Microtropis

Pi 0.0242

MarkerSeek score 66.40

Primer available yes

Label trnM-CAU-atpE

Feature Metadata

label_name: trnM-CAU-atpE
feature_type: igs
region: LSC
length_bp: 165
variable_sites: 4
pi: 0.0242
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-atpE_p1	GGCGAGAGTCATTGGTTCAA	AGAGGCAAACAATTGAGGCA	50.0	45.0	58.2	58.0	277 bp	1.0	79.6
trnM-CAU-atpE_p2	GGCGAGAGTCATTGGTTCAA	TTGAGGCAAATCTCGCTCTC	50.0	50.0	58.2	58.0	265 bp	1.0	79.5
trnM-CAU-atpE_p3	GGCGAGAGTCATTGGTTCAA	CTCTCAGACGAGCTAGGACA	50.0	55.0	58.2	58.0	250 bp	1.0	79.5
trnM-CAU-atpE_p4	GGCGAGAGTCATTGGTTCAA	ACTTGAGGAAAGCTGAAGGC	50.0	50.0	58.2	58.1	298 bp	1.0	78.8
trnM-CAU-atpE_p5	GGCGAGAGTCATTGGTTCAA	TGAGGAAAGCTGAAGGCAAG	50.0	50.0	58.2	58.1	295 bp	1.0	78.8