Hotspot

trnT-UGU-trnL-UAA

LSC · 50006-51136 · 1131 bp

Back to Microtropis

Pi 0.0167

MarkerSeek score 72.90

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 1131
variable_sites: 18
pi: 0.0167
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	50.0	50.0	57.8	58.0	1177-1213 bp	1.0	82.9
trnT-UGU-trnL-UAA_p2	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	50.0	50.0	57.8	58.1	1185-1221 bp	1.0	82.3
trnT-UGU-trnL-UAA_p3	GGAATCGAACCGATGACCAT	AGTCCGTAGCGTCTACCAAT	50.0	50.0	57.8	58.2	1191-1227 bp	1.0	80.9
trnT-UGU-trnL-UAA_p4	GGAATCGAACCGATGACCAT	TAAGTCCGTAGCGTCTACCA	50.0	50.0	57.8	57.6	1193-1229 bp	1.0	79.6
trnT-UGU-trnL-UAA_p5	GGAATCGAACCGATGACCAT	AAGTCCGTAGCGTCTACCAA	50.0	50.0	57.8	58.5	1192-1228 bp	1.0	79.1